isirv Antiviral Group
Teaching Resources
The PowerPoint presentations and photographs provided below can be downloaded and used for personal and educational use. We respectfully request that you do not alter the content, and acknowledge isirv-AVG when you use these resources.
If you have any queries regarding appropriate use, please contact us.
Influenza Antivirals: Challenges and Future Directions – Professor Fred Hayden 
This presentation was given at the Health Protection Agency Pandemic Influenza Conference, June 2010. It includes observations on the use of neuraminidase inhibitors during the 2009 influenza A(H1N1) pandemic, and details of investigational studies underway and future prospects for influenza antiviral agents.
Establishing surveillance for Neuraminidase Inhibitor Resistance - Professor Maria Zambon 
This presentation was given at a ViRgil training course in October 2006. It describes the points to consider when establishing a surveillance system for NI resistance, including choice of assay and analysis and interpretation of data.
Comparison of Neuraminidase Inhibition Assay Methodology - Professor Maria Zambon
This slide was presented at a ViRgil training course in October 2006. It provides a simple comparison of the chemiluminescent and fluorometric assay methodology.
Micro-plaque assay of influenza virus sensitivity to NAI – Dr Mikhail Matrosovich
This presentation was given at a ViRgil training course in October 2006. It describes the plaque reduction assay methodology and provides guidance on interpretation of results.
Amantadine Resistance – Dr Alan Hay
This presentation was given at a ViRgil training course in October 2006. It describes the mechanism of action of amantadine and the emergence and characterisation of amantadine resistant influenza viruses.
Mechanisms of Resistance – Dr Bruno Lina
This presentation was given at a ViRgil training course in October 2006. It describes the commonly occurring resistance mutants, including data on transmissibility. It also provides an overview on the epidemiology and surveillance of resistance.
This presentation was given at the ‘Options for the Control of Influenza’ conference in Toronto, 2007. It describes the replication of two strains of the influenza A H5N1 virus in the respiratory tract and internal organs of ferrets and the efficacy of oseltamivir treatment.
Surface Structure of Neuraminidase Heads
This graphical representation was provided by Dr Jennifer McKimm-Breschkin of the Commonwealth Scientific and Industrial Research Organisation (CSIRO).
It shows the structure of the tetramer NA ‘heads’, showing the 4-corners and the central ‘hole’, which can be seen in the electronmicrographs below.
Photographs 
Electron-micrographs of 2-dimensional arrays of neuraminidase ‘heads’ (kindly provided by Dr Jennifer McKimm-Breschkin of CSIRO)
